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Fig 1.

Map of Papua New Guinea and the Daru region.

(A) Papua New Guinea with the black box indicating the Daru region; (B) Inset of the Daru region from where the five Glyphis specimens were caught by fishers from the village of Katatai. Black star indicates the approximate capture location of the pregnant female Glyphis glyphis.

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Fig 2.

Fresh fins of the adult female Glyphis glyphis.

Field code 220348, estimated length 237–260 cm: (A) first dorsal fin; (B) right pelvic fin; (C) second dorsal fin; (D) lower caudal-fin lobe; (E) left pectoral fin; (F) right pectoral fin. Tape measure is set at 47 cm.

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Fig 3.

Whole jaw of Glyphis glyphis.

Adult female (CSIRO H 7670–01).

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Fig 4.

Dried first dorsal fin of Glyphis garricki.

Field code 220358, estimated length 100–105 cm.

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Fig 5.

Freshly caught adult males of Glyphis glyphis.

(A) estimated length 251–256 cm, caught 3rd Nov. 2014; (B) ~228 cm TL, caught 13 November 2014.

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Fig 6.

Freshly caught specimen of Glyphis garricki.

Estimated length 100–105 cm, caught 6 November 2014.

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Fig 7.

Molecular species identification using the mitochondrial NADH2 gene.

Neighbour-joining tree of NADH2 sequences, estimated using the Kimura 2 parameter distance model of molecular evolution, of Glyphis garricki, Glyphis glyphis, Glyphis gangeticus and Lamiopsis tephrodes. The species identifications of samples GN15749 and GN16686 are confirmed as G. glyphis and sample GN16684 is confirmed as G. garricki.

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Table 1.

Summary information on the five Papua New Guinea Glyphis specimens.

Date of observation, genetic sample id, location, sex, total length (TL; lengths estimated from fin measurements in parantheses), and key measurements of the first dorsal and pectoral fins (in cm) of the Glyphis specimens recorded from the Daru region.

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Fig 8.

Spines from the connective tissue of the Glyphis glyphis jaw.

Examples of spines found during dissection of the adult female jaw: (A) a stingray spine; (B) and (C), bony fish spines.

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